ÖлªÈËÃñ¹²ºÍ¹ú¹ú¼Ò±ê×¼ ÖлªÈËÃñ¹²ºÍ¹ú¹ú¼Ò±ê×¼

ʳƷÖÐïӵIJⶨ·½·¨¡¡¡¡¡¡¡¡¡¡¡¡¡¡GB 5009.15-85

Method for determination of cadmium in foods

©¥©¥©¥©¥©¥©¥©¥©¥©¥©¥©¥©¥©¥©¥©¥©¥©¥©¥©¥©¥©¥©¥©¥©¥©¥©¥©¥©¥©¥©¥©¥©¥©¥©¥©¥©¥©¥©¥©¥

±¾±ê×¼ÊÊÓÃÓÚ¸÷ÀàʳƷÖÐïӵIJⶨ¡£

µÚÒ»·¨¡¡Ô­×ÓÎüÊÕ·Ö¹â¹â¶È·¨

(µâ»¯¼Ø-4-¼×»ùÎìͪ-2·¨)

1¡¡Ô­Àí

ÑùÆ·¾­´¦Àíºó£¬ÔÚËáÐÔÈÜÒºÖÐïÓÀë×ÓÓëµâÀë×ÓÐγÉÂçºÏÎ²¢¾­4-¼×»ùÎìͪ-2ÝÍÈ¡·ÖÀ룬µ¼ÈëÔ­×ÓÎüÊÕÒÇÖУ¬Ô­×Ó»¯ÒÔºó£¬ÎüÊÕ228.8nm¹²ÕñÏߣ¬ÆäÎüÊÕÁ¿ÓëïÓÁ¿³ÉÕý±È£¬Óë±ê׼ϵÁбȽ϶¨Á¿¡£

2¡¡ÊÔ¼Á

ÒªÇóʹÓÃÈ¥Àë×ÓË®£¬Óż¶´¿»ò¸ß¼¶´¿ÊÔ¼Á¡£

2.1 1:10Á×Ëá

2.2¡¡1NÑÎË᣺Á¿È¡10mLÑÎËá¼ÓˮϡÊÍÖÁ120mL¡£

2.3 5NÑÎË᣺Á¿È¡50mLÑÎËá¼ÓˮϡÊÍÖÁ120mL¡£

2.4¡¡»ìºÏËá: ÏõËáÓë¸ßÂÈËá°´3:1»ìºÏ¡£

2.5¡¡1:1ÁòËá¡£

2.6 25£¥µâ»¯¼ØÈÜÒº¡£

2.7¡¡ïÓ±ê×¼ÈÜÒº£º¾«ÃܳÆÈ¡1.0000g½ðÊôïÓ(99.99£¥)£¬ÈÜÓÚ20mL5NÑÎËáÖÐ, ¼ÓÈë2µÎÏõËáºó£¬ÒÆÈë1000mLÈÝÁ¿Æ¿ÖУ¬ÒÔˮϡÊÍÖÁ¿Ì¶È£¬»ìÔÈ¡£ÖüÓÚ¾ÛÒÒÏ©Æ¿ÖС£´ËÈÜҺÿºÁÉýÏ൱ÓÚ1mgïÓ¡£

2.8 ïÓ±ê׼ʹÓÃÒº£ºÎüÈ¡10.0mLïÓ±ê×¼ÈÜÒº,ÖÃÓÚ100mLÈÝÁ¿Æ¿ÖÐ,ÒÔ1NÑÎËáÏ¡ÊÍÖÁ¿Ì¶È£¬»ìÔÈ¡£Èç´Ë¶à´ÎÏ¡ÊÍÖÁÿºÁÉýÏ൱ÓÚ0.2¦ÌgïÓ¡£

2.9 4-¼×»ùÎìͪ-2(MIBK,ÓÖÃû¼×»ùÒ춡ͪ)¡£

3 ÒÇÆ÷

Ô­×ÓÎüÊÕ·Ö¹â¹â¶È¼Æ¡£

4¡¡²Ù×÷·½·¨

4.1¡¡ÑùÆ·´¦Àí

4.1.1 ¹ÈÀࣺȥ³ýÆäÖÐÔÓÎï¼°³¾ÍÁ£¬±ØҪʱ³ýÈ¥Íâ¿Ç£¬ÄëË飬¹ý30Ŀɸ£¬»ìÔÈ¡£³ÆÈ¡5.0¡«10.0gÖÃÓÚ50mL´ÉÛáÛöÖУ¬Ð¡»ðÌ¿»¯£¬È»ºóÒÆÈë¸ßίÖУ¬500¡æÒÔÏ»һ¯Ô¼16hºó,È¡³öÛáÛö£¬·ÅÀäºóÔÙ¼ÓÉÙÁ¿»ìºÏËᣬС»ð¼ÓÈÈ£¬²»Ê¹¸ÉºÔ£¬±ØҪʱÔÙ¼ÓÉÙÐí»ìºÏËᣬÈç´Ë·´¸´´¦Àí£¬Ö±ÖÁ²ÐÔüÖÐÎÞÌ¿Á££¬´ýÛáÛöÉÔÀ䣬¼Ó10mL1NÑÎËᣬÈܽâ²ÐÔü²¢ÒÆÈë50mLÈÝÁ¿Æ¿ÖУ¬ÔÙÓÃ1NÑÎËá·´¸´Ï´µÓÛáÛö£¬Ï´Òº²¢ÈëÈÝÁ¿Æ¿ÖУ¬²¢Ï¡ÊÍÖÁ¿Ì¶È£¬»ìÔȱ¸Óá£È¡Óë´¦ÀíÑùÆ·ÏàͬÁ¿µÄ»ìºÏËáºÍ1NÑÎËᰴͬһ²Ù×÷·½·¨×öÊÔ¼Á¿Õ°×ÊÔÑé¡£

4.1.2 ÊßÀ³¡¢¹Ï¹û¼°¶¹Àà: È¡¿Éʳ²¿·ÖÏ´¾»ÁÀ¸É£¬³ä·ÖÇÐËé»ìÔÈ¡£³ÆÈ¡10.0¡«20.0gÖÃÓÚ´ÉÛáÛöÖÐ, ¼Ó1mL1:10Á×ËᣬС»ðÌ¿»¯£¬ÒÔÏ°´4.1.1×Ô¡°È»ºóÒÆÈë¸ßίÖС±ÆðÒÀ·¨²Ù×÷¡£

4.1.3 ÇÝ¡¢µ°¡¢Ë®²ú¼°ÈéÖÆÆ·£ºÈ¡¿Éʳ²¿·Ö³ä·Ö»ìÔÈ¡£³ÆÈ¡5.0¡«10.0gÖÃÓÚ´ÉÛáÛöÖУ¬Ð¡»ðÌ¿»¯£¬ÒÔÏ°´4.1.1×Ô¡°È»ºóÒÆÈë¸ßίÖС±ÆðÒÀ·¨²Ù×÷¡£ÈéÀྭ»ìÔȺó£¬Á¿È¡50mL£¬ÖÃÓÚ´ÉÛáÛöÖУ¬¼Ó1mL1:10Á×ËᣬÔÚˮԡÉÏÕô¸É£¬ÔÙС»ðÌ¿»¯£¬ÒÔÏ°´4.1.1×Ô¡°È»ºóÒÆÈë¸ßίÖС±ÆðÒÀ·¨²Ù×÷¡£

4.2¡¡ÝÍÈ¡·ÖÀë

ÎüÈ¡25mLÉÏÊöÖƱ¸µÄÑùÒº¼°ÊÔ¼Á¿Õ°×Òº,·Ö±ðÖÃÓÚ125mL·ÖҺ©¶·ÖÐ,¼Ó10mL1:1ÁòËᣬÔÙ¼Ó10mLË®£¬»ìÔÈ¡£ÎüÈ¡0.00¡¢0.25¡¢0.50¡¢1.50¡¢2.50¡¢3.50¡¢5.0mLïÓ±ê׼ʹÓÃÒº(Ï൱0¡¢0.05¡¢0.1¡¢0.3¡¢0.5¡¢0.7¡¢1.0¦ÌgïÓ), ·Ö±ðÖÃÓÚ125mL·ÖҺ©¶·ÖÐ, ¸÷¼Ó1NÑÎËáÖÁ25mL, ÔÙ¼Ó10mL1:1ÁòËá¼°10mLË®,»ìÔÈ¡£ÓÚÑùÆ·ÈÜÒº¡¢ÊÔ¼Á¿Õ°×Òº¼°ïÓ±ê×¼ÈÜÒºÖи÷¼Ó10mL25%µâ»¯¼ØÈÜÒº,»ìÔÈ, ¾²ÖÃ5min,ÔÙ¸÷¼Ó10mL4-¼×»ùÎìͪ-2ÕñÒ¡2min, ¾²Ö÷ֲãÔ¼0.5h,ÆúȥϲãË®Ïà,ÒÔÉÙÐíÍÑÖ¬ÃÞÈûÈë·ÖҺ©¶·Ï¾±²¿, ½«4-¼×»ùÎìͪ-2²ã¾­ÍÑÖ¬ÃÞÂËÖÁ10mL¾ßÈûÊÔ¹ÜÖÐ, ±¸Óá£

4.3 ²â¶¨

½«ÓлúÏർÈë»ðÑæ½øÐвⶨ, ²â¶¨Ìõ¼þ: µÆµçÁ÷6¡«7mA, ²¨³¤228.8nm, ÏÁ·ì0.15¡«0.2nm,¿ÕÆøÁ÷Á¿5L/min,ÒÒȲÁ÷Á¿0.4L/min, µÆÍ·¸ß¶È1, 뮵Ʊ³¾°Ð£Õý(Ò²¿É¸ù¾ÝÒÇÆ÷ÐͺÅ,µ÷ÖÁ×î¼ÑÌõ¼þ),ÒÔïÓº¬Á¿¶ÔӦŨ¶ÈÎü¹â¶È, »æÖƱê×¼ÇúÏ߱Ƚϡ£

4.4 ¼ÆËã

(A1-A2)¡Á1000

X1 = ©¤©¤©¤©¤©¤©¤©¤©¤©¤©¤ .................(1)

m1¡ÁV2/V1¡Á1000

ʽÖÐ: X1??ÑùÆ·ÖÐïӵĺ¬Á¿,mg/kg»òmg/L;

A1??²â¶¨ÓÃÑùÆ·ÒºÖÐïӵĺ¬Á¿, ¦Ìg;

A2??ÊÔ¼Á¿Õ°×ÒºÖÐïӵĺ¬Á¿, ¦Ìg;

m1??ÑùÆ·ÖÊÁ¿(Ìå»ý),g(mL);

V1??ÑùÆ·´¦ÀíÒºµÄ×ÜÌå»ý,mL;

V2??²â¶¨ÓÃÑùÆ·´¦ÀíÒºµÄÌå»ý,mL¡£

µÚ¶þ·¨ Ô­×ÓÎüÊÕ·Ö¹â¹â¶È·¨

(Ë«Áòëê-ÒÒËᶡõ¥·¨)

5 Ô­Àí

ÑùÆ·¾­´¦Àíºó,ÔÚpH6×óÓÒµÄÈÜÒºÖÐ, ïÓÀë×ÓÓëË«ÁòëêÐγÉÂçºÏÎï, ²¢¾­ÒÒËᶡõ¥ÝÍÈ¡·ÖÀë, µ¼ÈëÔ­×ÓÎüÊÕÒÇÖÐ, Ô­×Ó»¯ÒÔºó, ÎüÊÕ228.8nm¹²ÕñÏß, ÆäÎüÊÕÁ¿ÓëïÓÁ¿³ÉÕý±È,Óë±ê׼ϵÁбȽ϶¨Á¿¡£

6 ÊÔ¼Á

ÒªÇóʹÓÃÈ¥Àë×ÓË®, Óż¶´¿»ò¸ß¼¶´¿ÊÔ¼Á¡£

6.1 »ìºÏËá: ͬ2.4¡£

6.2 2MÄûÃÊËáÄÆ»º³åÒº: ³ÆÈ¡226.3gÄûÃÊËáÄƼ°48.46gÄûÃÊËá,¼ÓË®Èܽâ, ±ØҪʱ, ¼ÓÎÂÖúÈÜ, ÀäÈ´ºó¼ÓˮϡÊÍÖÁ500mL¡£ÁÙÓÃÇ°ÓÃ0.1%Ë«Áòëê-ÒÒËᶡõ¥ÈÜÒº´¦ÀíÒÔ½µµÍ¿Õ°×Öµ¡£

6.3 0.1%Ë«Áòëê-ÒÒËᶡõ¥ÈÜÒº: ³ÆÈ¡0.1gË«Áòëê, ¼Ó10mLÈýÂȼ×ÍéÈܽâºó, ÔÙ¼ÓÒÒËᶡõ¥Ï¡ÊÍÖÁ100mL, ÁÙÓÃʱÅäÖÆ¡£

6.4¡¡°±Ë®¡£

6.5 ïÓ±ê׼ʹÓÃÈÜÒº£ºÍ¬2.8¡£

7¡¡ÒÇÆ÷

Ô­×ÓÎüÊÕ·Ö¹â¹â¶È¼Æ¡£

8¡¡²Ù×÷·½·¨

8.1¡¡ÑùÆ·´¦Àí

¹ÈÀࣺȥ³ýÆäÖÐÔÓÎï¼°³¾ÍÁ£¬±ØҪʱ£¬³ýÈ¥Íâ¿Ç¡£

Ê߲ˡ¢¹Ï¹ûÀࣺȡ¿Éʳ²¿·ÖÏ´¾»ÁÀ¸É£¬ÇÐËé³ä·Ö»ìÔÈ¡£

ÈâÀàʳƷ£ºÈ¡¿Éʳ²¿·Ö£¬ÇÐËé³ä·Ö»ìÔÈ¡£

³ÆÈ¡5gÉÏÊöÑùÆ·£¬ÖÃÓÚ250mL¸ßÐÍÉÕ±­ÖУ¬¼Ó15mL»ìºÏËá, ¸ÇÉϱíÃæÃ󣬷ÅÖùýÒ¹£¬ÔÙÓÚµçÈÈ°å»òÉ°Ô¡ÉϼÓÈÈÏû»¯£¬Ïû»¯¹ý³ÌÖУ¬×¢ÒâÎðʹ¸ÉºÔ£¬±ØҪʱÔÙ¼ÓÉÙÁ¿ÏõËᣬֱÖÁÈÜÒº³ÎÃ÷ÎÞÉ«»ò΢´ø»ÆÉ«¡£Àäºó¼Ó25mLË®Öó·Ð£¬³ýÈ¥²ÐÓàµÄÏõËáÖÁ²úÉú´óÁ¿°×ÑÌΪֹ£¬Èç´Ë´¦ÀíÁ½´Î£¬·ÅÀä¡£ÒÔ25mLË®·Ö´Î½«ÉÕ±­ÄÚÈÝÎïÒÆÈë125mL·ÖҺ©¶·ÖС£È¡Óë´¦ÀíÑùÆ·ÏàͬÁ¿µÄ»ìºÏËᣬÏõËᰴͬһ²Ù×÷·½·¨×öÊÔ¼Á¿Õ°×ÊÔÑé¡£

8.2¡¡ÝÍÈ¡·ÖÀë

ÎüÈ¡0¡¢0.25¡¢0.50¡¢1.50¡¢2.50¡¢3.50¡¢5.0mLïÓ±ê׼ʹÓÃÒº(Ï൱0¡¢0.05¡¢0.1¡¢0.3¡¢0.5¡¢0.7¡¢1.0¦ÌgïÓ), ·Ö±ðÖÃÓÚ125mL·ÖҺ©¶·ÖÐ, ¸÷¼Ó1NÑÎËáÖÁ25mL¡£ÓÚÑùÆ·´¦ÀíÈÜÒº¡¢ÊÔ¼Á¿Õ°×Òº¼°ïÓ±ê×¼ÈÜÒº¸÷·ÖҺ©¶·Öи÷¼Ó5mL2MÄûÃÊËáÄÆ»º³åÒº,ÒÔ°±Ë®µ÷½ÚpHÖÁ5¡«6.4, È»ºó¸÷¼ÓË®ÖÁ50mL, »ìÔÈ¡£ÔÙ¸÷¼Ó5.0mL0.1%Ë«Áòëê-ÒÒËᶡõ¥ÈÜÒº,ÕñÒ¡2min, ¾²Ö÷ֲã, ÆúȥϲãË®Ïà, ½«Óлú²ã·ÅÈë¾ßÈûÊÔ¹ÜÖÐ, ±¸Óá£

8.3 ²â¶¨

ͬ4.3¡£

8.4 ¼ÆËã

(A3-A4)¡Á1000

X2 = ©¤©¤©¤©¤©¤©¤©¤©¤©¤©¤ ................(2)

m2¡Á1000

ʽÖÐ:X2??ÑùÆ·ÖÐïӵĺ¬Á¿,mg/kg;

A3??²â¶¨ÓÃÑùÆ·ÖÐïӵĺ¬Á¿,¦Ìg;

A4??ÊÔ¼Á¿Õ°×ÒºÖÐïӵĺ¬Á¿,¦Ìg;

m2??ÑùÆ·ÖÊÁ¿, g¡£

µÚÈý·¨ ±ÈÉ«·¨

9 Ô­Àí

ÑùÆ·¾­Ïû»¯ºó, ÔÚ¼îÐÔÈÜÒºÖÐïÓÀë×ÓÓë6-äå±½²¢àçßòżµªÝÁ·ÓÐγɺìÉ«ÂçºÏÎï, ÈÜÓÚ

ÈýÂȼ×Íé, Óë±ê׼ϵÁбȽ϶¨Á¿¡£

10 ÊÔ¼Á

10.1 40%¾ÆʯËá¼ØÄÆÈÜÒº¡£

10.2 20%ÇâÑõ»¯ÄÆÈÜÒº¡£

10.3 25%ÄûÃÊËáÄÆÈÜÒº¡£

10.4¡¡»ìºÏË᣺ÏõËáÓë¸ßÂÈËá°´3:1»ìºÏ¡£

10.5 ÈýÂȼ×Íé¡£

10.6 ïÓ±ê×¼ÈÜÒº£ºÍ¬2.7¡£

10.7 ïÓ±ê׼ʹÓÃÒº£ºÍ¬2.8£¬µ«Ï¡ÊÍÖÁÿºÁÉýÏ൱ÓÚ1¦ÌgïÓ¡£

10.8 ïÓÊÔ¼Á£º³ÆÈ¡38.4mg6-äå±½²¢àçßòżµªÝÁ·Ó£¬ÈÜÓÚ50mL¶þ¼×»ù¼×Ëá°·£¬ÖüÓÚ×ØÉ«

Æ¿ÖС£

11¡¡ÒÇÆ÷

¡¡¡¡·Ö¹â¹â¶È¼Æ¡£¡¡

12 ²Ù×÷·½·¨

12.1 ÑùÆ·Ïû»¯

³ÆÈ¡5.0¡«10.0gÑùÆ·£¬ÖÃÓÚ150mL׶ÐÎÆ¿ÖУ¬¼ÓÈë15¡«20mL»ìºÏËá(ÈçÔÚÊÒηÅÖùýÒ¹£¬Ôò´ÎÈÕÒ×ÓÚÏû»¯)£¬Ð¡»ð¼ÓÈÈ£¬´ýÅÝÄ­Ïûʧºó£¬¿ÉÂýÂý¼Ó´ó»ðÁ¦£¬±ØҪʱÔÙ¼ÓÉÙÁ¿ÏõËᣬֱÖÁÈÜÒº³ÎÇåÎÞÉ«»ò΢´ø»ÆÉ«£¬ÀäÈ´ÖÁÊÒΡ£È¡ÓëÏû»¯ÑùÆ·ÏàͬÁ¿µÄ»ìºÏËá¡¢ÏõËᰴͬһ²Ù×÷·½·¨×öÊÔ¼Á¿Õ°×ÊÔÑé¡£

12.2 ²â¶¨

½«Ïû»¯ºÃµÄÑùÒº¼°ÊÔ¼Á¿Õ°×ÒºÓÃ20mLË®·Ö´ÎÏ´Èë125mL·ÖҺ©¶·ÖУ¬ÒÔ20£¥ÇâÑõ»¯ÄÆÈÜÒºµ÷½ÚÖÁpH7×óÓÒ¡£ÎüÈ¡0.0¡¢0.5¡¢1.0¡¢3.0¡¢5.0¡¢7.0¡¢10.0mLïÓ±ê׼ʹÓÃÒº£¨Ï൱0¡¢0.5¡¢1¡¢3¡¢5¡¢7¡¢10¦ÌgïÓ£©£¬·Ö±ðÖÃÓÚ125mL·ÖҺ©¶·ÖÐ, ÔÙ¸÷¼ÓË®ÖÁ20mL¡£ÓÃ20%ÇâÑõ»¯ÄÆÈÜÒºµ÷½ÚÖÁpH7×óÓÒ¡£ÓÚÑùÆ·Ïû»¯Òº¡¢ÊÔ¼Á¿Õ°×Òº¼°ïÓ±ê×¼ÒºÖи÷ÒÀ´Î¼ÓÈë3mL25%ÄûÃÊËáÄÆÈÜÒº¡¢4mL40%¾ÆʯËá¼ØÄÆÈÜÒº¼°1mL20%ÇâÑõ»¯ÄÆÈÜÒº, »ìÔÈ¡£ÔÙ¸÷¼Ó5.0mLÈýÂȼ×Íé¼°0.2mLïÓÊÔ¼Á, Á¢¼´ÕñÒ¡2min, ¾²Ö÷ֲãºó, ½«ÈýÂȼ×Íé²ã¾­ÍÑÖ¬ÃÞÂËÓÚ1cm±ÈÉ«±­ÖÐ, ÒÔÁã¹Üµ÷½ÚÁãµã,ÓÚ²¨³¤585nm´¦²âÎü¹â¶È, »æÖƱê×¼ÇúÏ߱Ƚϡ£

12.3 ¼ÆËã

ͬ8.4¡£

¡¡¡¡¡¡¡¡¡¡¡¡¡¡¡¡¡¡¡¡¡¡¡¡¡¡©¥©¥©¥©¥©¥©¥©¥©¥©¥

¸½¼Ó˵Ã÷£º

±¾±ê×¼ÓÉÈ«¹úÎÀÉú±ê×¼¼¼ÊõίԱ»áʳƷÎÀÉú±ê×¼·ÖίԱ»áÌá³ö£¬ÓÉÎÀÉú²¿Ê³Æ·ÎÀ

Éú¼à¶½¼ìÑéËù¹é¿Ú¡£

±¾±ê×¼µÚÒ»¡¢¶þ·¨ÓÉÉϺ£ÊÐÎÀÉú·ÀÒßÕ¾¡¢ÖйúÔ¤·ÀҽѧÖÐÐÄÎÀÉúÑо¿Ëù¸ºÔðÆð²Ý¡£

±¾±ê×¼µÚÈý·¨Óɽ­ËÕÊ¡ÎÀÉú·ÀÒßÕ¾¸ºÔðÆð²Ý¡£

©¥©¥©¥©¥©¥©¥©¥©¥©¥©¥©¥©¥©¥©¥©¥©¥©¥©¥©¥©¥©¥©¥©¥©¥©¥©¥©¥©¥©¥©¥©¥©¥©¥©¥©¥©¥©¥©¥©¥

ÖлªÈËÃñ¹²ºÍ¹úÎÀÉú²¿1985-05-16·¢²¼ ¡¡¡¡ 1985-12-01ʵʩ