對不同品種蘿卜澱粉酶活性的研究
womendouzhidaoluobushishuyuhuakeleide,tayoujiaolailv,shiwomenshiyongdeshucaizhongbijiaochangjiandeyizhongzhiyi,tadeshengchangshiyingxingnengshibijiaogaode,meiniandechanliangyeshixiangdangkeguande,xiannendeguoroushihanyouwomenrenleimeitiansuoxuyaodeyingyangchengfende,beiwomendajiachengzhiweidongjiderencan。yinweiluobuhanyoufengfudedianfenmei,shiyizhongreliangbijiaodideshucai,jutidedianfenmeihanliangwomentongguo直鏈澱粉含量儀來指導具體的數值。由於其含富含澱粉酶,因此對胃部粘膜的修複具有很好的促進作用,能夠防止胃酸過多、胃(wei)炎(yan)及(ji)胃(wei)潰(kui)瘍(yang),同(tong)時(shi)能(neng)夠(gou)增(zeng)強(qiang)消(xiao)化(hua)機(ji)能(neng)。因(yin)此(ci),蘿(luo)卜(bu)一(yi)直(zhi)以(yi)來(lai)都(dou)被(bei)認(ren)為(wei)是(shi)有(you)助(zhu)消(xiao)化(hua)的(de)食(shi)品(pin)。但(dan)是(shi)有(you)關(guan)蘿(luo)卜(bu)澱(dian)粉(fen)酶(mei)的(de)研(yan)究(jiu)甚(shen)少(shao),因(yin)此(ci),該(gai)試(shi)驗(yan)以(yi)不(bu)同(tong)蘿(luo)卜(bu)類(lei)型(xing)品(pin)係(xi)及(ji)組(zu)合(he)為(wei)試(shi)材(cai),對(dui)不(bu)同(tong)蘿(luo)卜(bu)品(pin)種(zhong)澱(dian)粉(fen)酶(mei)活(huo)性(xing)進(jin)行(xing)比(bi)較(jiao)研(yan)究(jiu),為(wei)進(jin)一(yi)步(bu)開(kai)展(zhan)蘿(luo)卜(bu)澱(dian)粉(fen)酶(mei)純(chun)化(hua)和(he)理(li)化(hua)性(xing)質(zhi)研(yan)究(jiu)及(ji)高(gao)澱(dian)粉(fen)酶(mei)活(huo)性(xing)品(pin)種(zhong)選(xuan)育(yu)提(ti)供(gong)基(ji)礎(chu)。
在國內有許多學者都運用3,5-二(er)硝(xiao)基(ji)水(shui)楊(yang)酸(suan)比(bi)色(se)法(fa)來(lai)研(yan)究(jiu)澱(dian)粉(fen)酶(mei)活(huo)性(xing),但(dan)是(shi)操(cao)作(zuo)過(guo)程(cheng)複(fu)雜(za)且(qie)嚴(yan)格(ge),為(wei)了(le)降(jiang)低(di)誤(wu)差(cha),該(gai)研(yan)究(jiu)在(zai)整(zheng)個(ge)試(shi)驗(yan)過(guo)程(cheng)中(zhong)將(jiang)樣(yang)品(pin),按(an)照(zhao)區(qu)組(zu)每(mei)次(ci)重(zhong)複(fu)為(wei)1組同時測定,共分3組(zu)同(tong)時(shi)利(li)用(yong)電(dian)腦(nao)定(ding)時(shi)精(jing)確(que)到(dao)每(mei)個(ge)樣(yang)品(pin)的(de)反(fan)應(ying)時(shi)間(jian),而(er)且(qie)測(ce)定(ding)時(shi)均(jun)設(she)重(zhong)複(fu),從(cong)多(duo)個(ge)角(jiao)度(du)較(jiao)少(shao)誤(wu)差(cha),並(bing)利(li)用(yong)統(tong)計(ji)分(fen)析(xi)排(pai)除(chu)外(wai)界(jie)誤(wu)差(cha),提(ti)高(gao)測(ce)定(ding)的(de)準(zhun)確(que)度(du)。該(gai)研(yan)究(jiu)結(jie)果(guo)證(zheng)明(ming)了(le)不(bu)同(tong)蘿(luo)卜(bu)品(pin)種(zhong)總(zong)澱(dian)粉(fen)酶(mei)活(huo)性(xing)、α澱粉酶活性和β澱粉酶活性的澱粉酶活性差異均顯著,也說明在遺傳上也存在著質的區別,也為進一步開展蘿卜α澱粉酶和 β澱粉酶的同工酶研究及澱粉酶純化和理化性質研究奠定了基礎。
α澱粉酶活性占總酶活性的百分比的變異範圍為8.11%~25.36%,而β澱粉酶活性占總酶活性的百分比的變異範圍為74.64%~91.89%。從二者所占比例可知, β澱粉酶占主導地位。標準麥芽糖溶液(1mg/mL):精確稱取100mg麥芽糖,用蒸餾水溶解並定容至100mL;3,5-二硝基水楊酸試劑:精確稱取1g3,5-二硝基水楊酸,溶於20mL 2mol/L NaOH溶液中,加入50mL蒸餾水,再加入30g酒石酸鉀鈉,待溶解後用蒸餾水定容至100 mL。蓋 緊 瓶 塞,勿 使CO2進 入;0.1 mol/LpH 5.6的檸檬酸緩衝液:A液(0.1mol/L檸檬酸):稱取C6H8O7 ・H2O 21.01g,用蒸餾水溶解至1L。B液(0.1mol/L檸檬酸鈉):稱取Na3C6H5O7 ・2H2O 29.41g用蒸餾水定容至1L。取A液55mL與B液145mL混勻,即為0.1mol/L pH 5.6的檸檬酸緩衝液;1%澱粉溶液:稱取1g澱粉溶於100mL 0.1mol/L pH 5.6的檸檬酸緩衝液中。中國糧油儀器網 http://www.98fo.cn/



