從葉綠素檢測儀的測量原理說起
zaizhiwuxibaozhong,yelvsucunzaiyelvtideleinangtimozhong,shizhiwujinxingguanghezuoyongdezhongyaowuzhi,xiangxindajiazaishengwukexuezhongyedouyousuorenshi。gaodengzhiwuzhongzhuyaohanyelvsuA和葉綠素B,在褐藻、矽藻類中含葉綠素A、C1、C2;紅藻中含葉綠素D等。為了有效區分出不同植物中的葉綠素含量,大多數研究人員會直接利用葉綠素檢測儀進行測量,快速準確。

如(ru)果(guo)還(hai)有(you)朋(peng)友(you)對(dui)葉(ye)綠(lv)素(su)檢(jian)測(ce)儀(yi)不(bu)夠(gou)了(le)解(jie)的(de)話(hua),我(wo)們(men)可(ke)以(yi)從(cong)它(ta)的(de)測(ce)量(liang)原(yuan)理(li)說(shuo)起(qi)。該(gai)儀(yi)器(qi)是(shi)根(gen)據(ju)葉(ye)綠(lv)體(ti)色(se)素(su)提(ti)取(qu)液(ye)對(dui)可(ke)見(jian)光(guang)譜(pu)的(de)吸(xi)收(shou),利(li)用(yong)分(fen)光(guang)光(guang)度(du)計(ji)在(zai)某(mou)一(yi)特(te)定(ding)波(bo)長(chang)測(ce)定(ding)其(qi)吸(xi)光(guang)度(du),即(ji)可(ke)用(yong)公(gong)式(shi)計(ji)算(suan)出(chu)提(ti)取(qu)液(ye)中(zhong)各(ge)色(se)素(su)的(de)含(han)量(liang)。根(gen)據(ju)朗(lang)伯(bo)―比爾定律,某有色溶液的吸光度A與其中溶質濃度C和液層厚度L成正比,即A=αCL式中:α比例常數。當溶液濃度以百分濃度為單位,液層厚度為1cm時,αweigaiwuzhidexiguangxishu。gezhongyousewuzhirongyezaibutongbochangxiadexiguangxishuketongguocedingyizhinongdudechunwuzhizaibutongbochangxiadexiguangduerqiude。ruguorongyezhongyoushuzhongxiguangwuzhi,zecihunheyezaimouyibochangxiadezongxiguangdudengyugezufenzaixiangyingbochangxiaxiguangdudezonghe。zhejiushixiguangdudejiahexing。jinyucedingyelvtisesuhunhetiquyezhongyelvsua、b和類胡蘿卜素的含量,隻需測定該提取液在三個特定波長下的吸光度A,並根據葉綠素a、b及類胡蘿卜素在該波長下的吸光係數即可求出其濃度。在測定葉綠素a、b時為了排除類胡蘿卜素的幹擾,所用單色光的波長選擇葉綠素在紅光區的最大吸收峰。
jiandanlaishuo,yelvsujianceyiyejiushizaiyelvsuxuanzexishoutedingbochangguangdelianggebochangquyu,genjuyepiantousheguangdelianglaijisuanchuyelvsuhanliangde。lingwai,genjuyelvsuhanliang,renmenkeyouxiaopanduanchuzuowuduidansudexuqiuliang,jiunenghelikongzhidanfeideshiyongliang,jianshaolangfei,zheyezhengshiliyongyiqiceliangdeyiyisuozai。
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